HIV-1 inhibits phagocytosis and inflammatory cytokine responses of human monocyte-derived macrophages to P. falciparum infected erythrocytes

LE Ludlow; J Zhou; E Tippett; WJ Cheng; W Hasang; SJ Rogerson; A Jaworowski

Journal article

HIV-1 inhibits phagocytosis and inflammatory cytokine responses of human monocyte-derived macrophages to P. falciparum infected erythrocytes

LE Ludlow, J Zhou, E Tippett, WJ Cheng, W Hasang, SJ Rogerson, A Jaworowski

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2012

DOI: 10.1371/journal.pone.0032102

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Abstract

HIV-1 infection increases the risk and severity of malaria by poorly defined mechanisms. We investigated the effect of HIV-1 Ba-L infection of monocyte-derived macrophages (MDM) on phagocytosis of opsonised P. falciparum infected erythrocytes (IE) and subsequent proinflammatory cytokine secretion. Compared to mock-infected MDM, HIV-1 infection significantly inhibited phagocytosis of IE (median (IQR) (10 (0-28) versus (34 (27-108); IE internalised/100 MDM; p = 0.001) and decreased secretion of IL-6 (1,116 (352-3,387) versus 1,552 (889-6,331); pg/mL; p = 0.0078) and IL-1β (16 (7-21) versus 33 (27-65); pg/mL; p = 0.0078). Thus inadequate phagocytosis and cytokine production may contribute to im..

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University of Melbourne Researchers

Louise Ludlow Author

Wina Hasang Author

Stephen Rogerson Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

Funding was provided by Australian NHMRC Project Grants 400090 and 628611 to AJ and SJR. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.